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e. coli t7 s30 extract system for circular dna  (Promega)

 
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    Promega e. coli t7 s30 extract system for circular dna
    E. Coli T7 S30 Extract System For Circular Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e%2E+coli+s30+extract+system+for+circular+dna/e++coli+t7+s30+extract+system+for+circular+dna/pm40593631-296-20-22
    Average 90 stars, based on 1 article reviews
    e. coli t7 s30 extract system for circular dna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: Exploration of the Fusidic Acid Structure Activity Space for Antibiotic Activity.
    Article Snippet: .. An E. coli S30 extract system for circular DNA (Promega, Madison, WI, USA) was used for coupled in vitro transcription–translation assays set up in 384-well black microplates (PHENIX Research, Swedesboro, NJ, USA). .. Nanoluciferase (NLuc, 100 ng) expression plasmid and compounds in concentrations ranging from 400 μM to 0.1 μM. in twofold dilutions were added to each reaction, followed by incubation at 37 ◦C for 60 min. After incubation, PBS containing a 1000-fold dilution of furimazine substrate (Nano-Glo Luciferase Assay System, Promega, Madison, WI, USA) was added to each well, and luminescence was measured using an Infinite M1000 Pro microplate reader (Tecan, Morrisville, NC, USA).

    Article Title: Design and structure-activity relationships of ether-linked alkylides: Hybrids of 3-O-descladinosyl macrolides and quinolone motifs.
    Article Snippet: Ketolides (3-keto) such as TE-802 and acylides (3-O-acyl) like TEA0929 are ineffective against constitutively resistant pathogens harboring erythromycin ribosomal methylation (erm) genes.. Following our previous work on alkylides (3-O-alkyl), we explored the structure–activity relationships of hybrids combining (R/S) 3-descladinosyl erythromycin with 6/7-quinolone motifs, featuring extended ether-linked spacers, with a focus on their efficacy against pathogens bearing constitutive erm gene resistance.. Optimized compounds 17a and 31f not only reinstated efficacy against inducibly resistant pathogens but also demonstrated significantly augmented activities against constitutively resistant strains of Streptococcus pneumoniae and Streptococcus pyogenes, which are typically refractory to existing C-3 modified macrolides.

    Article Title: Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.
    Article Snippet: Nourseothricin, S-D, and S-F were distributed to a 384-well black microplates (PHENIX Research, Swedesboro, NJ). .. For bacterial in vitro translation assays, E. coli S30 Extract System for Circular DNA (Promega, #L1020) and 100 ng of pCR XL TOPO-T7NLuc were then added to each reaction well, making use of the upstream vector, pLac promoter rather than the cloned T7 promoter for expression in the current studies. .. After incubation at 37 ̊C for 60 minutes, an equal volume of PBS buffer with 1 μg/mL Nluc-specific furimazine substrate (AOBIOUS, Gloucester, MA) was added to each well, and luminescent signal was detected with an Infinite M1000 Pro (TECAN, Morrisville, NC) plate reader.

    other:

    Article Title: Characterization of Dichloroisoeverninic Acid Biosynthesis and Chemoenzymatic Synthesis of New Orthosomycins.
    Article Snippet: A commercial cell-free transcription/translation assay kit (E. coli S30 Extract System for Circular DNA, Promega) was used to measure in vitro ribosomal inhibition of everninomicin compounds, using a luciferase gene (pBESTluc, Promega) as a reporter of protein synthesis.

    Article Title: Characterization of Five Purine Riboswitches in Cellular and Cell-Free Expression Systems.
    Article Snippet: Plasmids were eluted with RNase-free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega).

    Plasmid Preparation:

    Article Title: Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.
    Article Snippet: Nourseothricin, S-D, and S-F were distributed to a 384-well black microplates (PHENIX Research, Swedesboro, NJ). .. For bacterial in vitro translation assays, E. coli S30 Extract System for Circular DNA (Promega, #L1020) and 100 ng of pCR XL TOPO-T7NLuc were then added to each reaction well, making use of the upstream vector, pLac promoter rather than the cloned T7 promoter for expression in the current studies. .. After incubation at 37 ̊C for 60 minutes, an equal volume of PBS buffer with 1 μg/mL Nluc-specific furimazine substrate (AOBIOUS, Gloucester, MA) was added to each well, and luminescent signal was detected with an Infinite M1000 Pro (TECAN, Morrisville, NC) plate reader.

    Clone Assay:

    Article Title: Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.
    Article Snippet: Nourseothricin, S-D, and S-F were distributed to a 384-well black microplates (PHENIX Research, Swedesboro, NJ). .. For bacterial in vitro translation assays, E. coli S30 Extract System for Circular DNA (Promega, #L1020) and 100 ng of pCR XL TOPO-T7NLuc were then added to each reaction well, making use of the upstream vector, pLac promoter rather than the cloned T7 promoter for expression in the current studies. .. After incubation at 37 ̊C for 60 minutes, an equal volume of PBS buffer with 1 μg/mL Nluc-specific furimazine substrate (AOBIOUS, Gloucester, MA) was added to each well, and luminescent signal was detected with an Infinite M1000 Pro (TECAN, Morrisville, NC) plate reader.

    Expressing:

    Article Title: Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.
    Article Snippet: Nourseothricin, S-D, and S-F were distributed to a 384-well black microplates (PHENIX Research, Swedesboro, NJ). .. For bacterial in vitro translation assays, E. coli S30 Extract System for Circular DNA (Promega, #L1020) and 100 ng of pCR XL TOPO-T7NLuc were then added to each reaction well, making use of the upstream vector, pLac promoter rather than the cloned T7 promoter for expression in the current studies. .. After incubation at 37 ̊C for 60 minutes, an equal volume of PBS buffer with 1 μg/mL Nluc-specific furimazine substrate (AOBIOUS, Gloucester, MA) was added to each well, and luminescent signal was detected with an Infinite M1000 Pro (TECAN, Morrisville, NC) plate reader.

    Article Title: Design of an intracellular aptamer-based fluorescent biosensor to track burden in Escherichia coli.
    Article Snippet: For the constructs under the control of the T7 promoter, expression was performed using the PURExpress® In Vitro Protein Synthesis Kit (NEB). .. For the non-T7 constructs, expression was performed using the E. coli S30 Extract System for Circular DNA (Promega). ..

    Construct:

    Article Title: Design of an intracellular aptamer-based fluorescent biosensor to track burden in Escherichia coli.
    Article Snippet: For the constructs under the control of the T7 promoter, expression was performed using the PURExpress® In Vitro Protein Synthesis Kit (NEB). .. For the non-T7 constructs, expression was performed using the E. coli S30 Extract System for Circular DNA (Promega). ..



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